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The development of competitive enzyme-linked immunosorbent assays (ELISAs) based on polyclonal antibodies for coplanar congeners is described. As a first step to developing the functional ELISAs, hapten mimics with different lengths of carboxylic acid spacers at para-position were derived from coplanar 3,3',4,4'-tetrachlorobiphenyl and 3,3',4,4',5-pentachlorobiphenyl (IUPAC No. 77 (PCB 77) and 126...
The syntheses of 4,4 -dichlorobiphenyl-and 3 ,4,4 ,5-tetrachlorobiphenyl-2-azo-protein immunogens and the corresponding haptens conjugated to peroxidase through the amidoadipate spacer arm are described. Only one sheep of the 24 immunized animals yielded an antiserum with superior antibody titres. The direct competitive enzyme-linked immunosorbent assay (ELISA) incorporating this antiserum showed...
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