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The design of a simple, highly miniaturized instrument for manual microsequence analysis of proteins and peptides is described. The reaction chamber is made of fused silica capillary tubing with all reagents and solvents necessary for coupling and cleavage delivered via two valves and a syringe-based dispenser. Only two pressure regulators are required. A section of the flow-through reaction chamber...
A miniaturized protein and peptide microsequencer consisting of a fused silica capillary reaction chamber is described. Extremely small volumes of reagents, 2 μl or less, were delivered directly to the reaction chamber through individual fused silica capillary lines using low pressure syringe pumps. Other than an argon gas controller, no valves were used in the delivery system. The short flow path...
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