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Incomplete labeling of proteins by a derivatizing reagent usually results in the formation of a large number of products, which can produce unacceptable band broadening during electrophoretic analysis. In this paper, we report on the reaction of the fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde (FQ) with the lysine residues of ovalbumin. Mass spectrometry was first used to determine the distribution...
The fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde (FQ) has proven valuable in the analysis of proteins and small neurotransmitters. We monitored the reaction rate between this reagent and five neurotransmitters at 40 o and 65 o C in artificial cerebrospinal fluid. The reactions followed pseudo-first order kinetics. The activation energy for the reaction of FQ was 10.6, 10...
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