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An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-β-galactosidase, followed by treatment with N-acetyl-β-glucosaminidase, yielding products which could be analysed by 1 H-NMR...
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