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An ultrasensitive electrochemical immunoassay method based on counting single magnetic nanobead (MNB) with combined amplification of nanobead and enzyme. Carcinoembryonic antigen (CEA) and MNB were initially immobilized on substrate at 1:1 molar ratio through sandwich immunoreactions. The MNBs were then labeled with alkaline phosphatase (AP) and continuously introduced to capillary with disodium phenyl...
An epi-fluorescence microscopy for determination of peroxidase in individual neutrophils was developed by a combination of enzyme-catalyzed reaction and fluorescence detection. In this method, an individual cell was transferred into a microliter-volume vessel and lysed by freeze-thawing and ultrasonication. The peroxidases-catalyzed reaction was initiated by adding the buffer solution containing nonfluorescent...
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