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Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI “Max”) regarding structure, polyplex formation with plasmid DNA, and transfection of suspension‐adapted HEK‐293E cells. PEI “Max” binds DNA more efficiently than the other PEIs, but it is the least effective in terms of transient recombinant protein yield. One PEI lot...
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