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The liver-specific toxin microcystin-LR (MC-LR) is a potent inhibitor of type 1 (PP1) and type 2A (PP2A) protein phosphatases. A tritiated form of the toxin, [ 3 H]dihydromicrocystin-LR ([ 3 H]DMC-LR), was used to identify target proteins in cellular fractions prepared from rat liver homogenates. About 80% of the [ 3 H]DMC-LR bound to proteins was in the cytosolic fraction,...
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