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Refolding of a mutant form of green fluorescent protein (eGFP), which only emits characteristic fluorescence when in the natively folded state, was accomplished under high hydrostatic pressure (HHP). Compression of eGFP inclusion bodies (IB) at 2.40kbar for 30min dissociated most of the aggregates and reduced the quantity of IBs. However, fluorescence at 509nm indicated that eGFP did not refold under...
The effects of hydrostatic pressure on the excited state reactions of the photosynthetic system of cyanobacteria were studied with the use of stationary and dynamic fluorescence spectroscopy. When the cells were excited with blue light (442 nm), hydrostatic pressure promoted a large increase in the fluorescence emission of the phycobilisomes (PBS). When PBS were excited at 565 nm, the shoulder originating...
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