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Chemokines (e.g., IL-8) were originally identified as chemotactic proteins obtained from various different natural sources. Today, using the genome walking strategy an ever-increasing number of novel genes encoding chemokines have been discovered that were expressed in bacteria or eukaryotic cells and subsequently tested for biological activity. Usually biological significance of the considered chemokine...
Eotaxin is a potent and selective CC chemokine for eosinophils and basophils. We established several monoclonal antibodies (Mabs) allowing the neutralization and measurement of human eotaxin. Using the Mabs as probes, we demonstrated that normal eosinophils contained intracellular granule-associated eotaxin. Quantification of cell-associated eotaxin in different leukocyte subsets revealed that...
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