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The Boyle–van’t Hoff (BVH) law of physics has been widely used in cryobiology for calculation of the key osmotic parameters of cells and optimization of cryo-protocols. The proper use of linearization of the Boyle–vant’Hoff relationship for the osmotically inactive volume (v b ) has been discussed in a rigorous way in (Katkov, Cryobiology, 2008, 57:142–149). Nevertheless, scientists in the...
Some aspects of proper linearization of the Boyle–van’t Hoff (BVH) relationship for calculation of the osmotically inactive volume vb, and Arrhenius plot (AP) for the activation energy Ea are discussed. It is shown that the commonly used determination of the slope and the intercept (vb), which are presumed to be independent from each other, is invalid if the initial intracellular molality m0 is known...
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