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Targeted proteomics depends on the availability of stable isotope labeled (SIL) peptide standards, which for absolute protein quantification need to be absolutely quantified. In the present study, three new approaches for absolute quantification of SIL peptides are developed. All approaches rely on a quantification tag (Qtag) with a specific UV absorption. The Qtag is attached to the peptide during...
DOI: 10.1002/pmic.202000007
Targeted proteomics, a powerful strategy for simultaneous determination of human proteins, requires quantified stable isotope labeled (SIL) peptide standards to enable precise protein quantification. In article number 2000007 by Karsten Schnatbaum et al., a new approach for absolute quantification of SIL peptides is reported, which eliminates the need for laborious and...
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