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We have developed and evaluated a next‐generation bisulfite sequencing (NGS) assay to distinguish HPV16 cervical precancer (CIN2–3; N =59) from HPV16‐positive transient infections (N = 40). Cervical DNA was isolated and treated with bisulfite and HPV16 methylation was quantified by (i) amplification with barcoded primers and massively parallel single molecule sequencing and (ii) site‐specific pyrosequencing...
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