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In situ observation of single‐molecule surface reactions from low to high affinities is achieved by resonant coupling between optical whispering‐gallery modes and the localized surface plasmon of nanorods. Transient and permanent interactions between ligands (thiol, amine) and the gold surface are monitored without labels, allowing direct determination of the associated kinetic constants and rapid...
A new optical approach resolves transient as well as permanent interactions of single molecules at the nano‐particle interface in situ, as reported on page 9941 by F. Vollmer and co‐workers. Reaction kinetics are monitored over a broad range of affinities and especially under conditions where no reaction product is formed on average. This enables the real time characterization of reactions during...
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