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Complementary DNA sequences encoding different portions of two sunflower homeodomain proteins were cloned in-frame in the expression vectors pRSET and pGEX-3X. When introduced into competentEscherichia colicells and induced, the resulting plasmids directed the expression of large amounts (5–10% of total cellular protein) of the encoded polypeptides. As a rule, fusions in pRSET rendered insoluble proteins,...
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