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Pyrophosphate (PPi), as a biologically related phosphate anion, plays very important roles in organisms. Here, a highly selective visual method for distinction of PPi was made with commercial available 4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene (5-Cl-PADAB) in the presence of copper(II). The yellow solution of 5-Cl-PADAB exhibits strong absorption at 450.5nm, and addition of Cu(II) results in...
Pyrophosphate (PPi) is involved in lots of anabolism and bioenergetic processes in organisms and possesses important biological functions so that its detection is very significant. Here, we developed a selective fluorometric detection method for PPi with a copper(II) complex of 2,6-bis(2-benzimidazolyl)pyridine (bbimp), and then applied it to the detection of bacterial alarmone ppGpp. bbimp has the...
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