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Shiraia bambusicola can biosynthesize the valuable hypocrellin, which is applied in antibacterial, antitumoral, and antiviral fields. The lack of an effective transformation system limits studies on the hypocrellin metabolism deconstruction. We successfully expressed a lentiviral PgfpHyg vector in the Shiraia sp. SUPER-H168 by a polyethylene glycol and CaCl2 transformation method. The transformant...
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