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AbstractA systematic study is performed about several factors involved in the measurements when a absorbent blank is used, as molar absorption coefficient of the formed complex, absorbance of the blank at the measured wavelength, and kind of spectrophotometer used (conventional double-beam or diode-array). It is proved that the usual methodology, measuring against reagent blank, produces less accurate...
The H-point standard additions method (HPSAM) is proposed to resolve overlapped peaks in liquid chromatography. The method uses the areas obtained at two previously selected wavelengths as analytical signals, and good results are obtained for highly overlapped peaks with highly overlapped spectra. The principal benefits of the method are the ease with which the required wavelengths can be selected,...
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