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A cDNA clone coding for the trypsin inhibitor MsTI from snail medic (Medicago scutellata L.) was isolated by RT-PCR using poly(A) + mRNA purified from immature seeds. A partial cDNA fragment was produced using degenerated oligonucleotides designed from the available MsTI amino acid sequence. The full-length cDNA was obtained by RACE-PCR experiments. A putative signal peptide for subcellular...
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