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The open reading frame of the α-subunit (amino acids 1–211) of human muscle nicotinic acetylcholine receptor (hAChR α211 ) was inserted into eukaryotic expression vector of pPIC9K to form a recombinant plasmid. After transformation and expression, the target protein rhAChR α211 (recombinant hAChR α211 ) was secretively expressed in recombinant strain P. pastoris GS115/pPIC9K–hAChR...
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