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We studied the structure of aorta heparan sulfate (HS) obtained from human autopsy material. HS from 13 subjects, aged 20-80 years, was isolated and treated with HNO 2 at lowp H. The resulting disaccharides were radio labeled and separated using anion exchange chromatography. We found that the content of 6-O-sulfated glucosamine residues was twice as high in HS from ≥60-year old subjects...
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