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Two engineered Escherichia coli strains, DQ101 (MG1655 fadD−)/pDQTES and DQ101 (MG1655 fadD−)/pDQTESZ were constructed to investigate the free fatty acid production using ionic liquid-based acid- or enzyme-catalyzed bamboo hydrolysate as carbon source in this study. The plasmid, pDQTES, carrying an acyl-ACP thioesterase ‘TesA of E. coli in pTrc99A was constructed firstly, and then (3R)-hydroxyacyl-ACP...
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