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The overproduction of androgens during the course of foliculogenesis may lead to disturbances in ovulation – the main reason for the establishment of the pathological state known as polycystic ovarian syndrome (PCOS). Furthermore, recently attention has been drawn to the involvement of tachykinins such as substance P (SP) in this process. Apart from this, both in animals and women, the above factors...
The aim of the study was to analyze the contribution of kisspeptin-10 (KiSS-10) and peptide 234 (kisspeptin-234, potent neutral antagonist of GPR-54 receptors) to the modulation of GnRH-induced folliclestimulating hormone (FSH) secretion from anterior pituitary cells of prepubertal ram lambs in vitro. Pituitary cells were cultured in McCoy 5A medium without hormones (the negative control), with GnRH...
The aim of the study was to investigate the effect of 2-methoxyestradiol (2-ME) on GnRH-induced LH and FSH secretion by porcine pituitary cells in vitro. Moreover, the concentrations of superoxide anion radical (O₂⁻), as a possible mediator of 2-ME action, and malonyldialdehyde (MDA), as an oxidative stress indicator, were estimated. Pituitary cells were cultured in McCoy 5A medium with GnRH (positive...
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