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Helicobacter pylori is a major cause of chronic gastritis, peptic ulcer disease, and associated with gastric carcinoma. H. pylori Neutrophil-activating protein (HP-NAP) is one of the major virulence factors. It has been shown that HP-NAP is a good candidate antigen for a multi-epitopes vaccine against H. pylori. However, the B-cell epitopes of HP-NAP are largely unknown. Bioinformatic and immunological...
The efficient tetravalent vaccine is urgently needed to provide long-term and all-round immunity against dengue virus. In order to construct the tetravalent attenuated vaccine, the well defined B-cell and T-cell epitopes of the glycoprotein of the DEN-1 HAWAII strain were predicted and analyzed by using network servers. The long RT-PCR was established to amplify the cDNA of DEN-2 NGC strain for constructing...
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