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The mechanism of theN 5,10 -methenyltetrahydrofolate synthetase reaction was probed by determining the source of the oxygen atom introduced between the β- and γ-phosphates as ATP is converted to ADP and P i . The reaction was performed using a mixture of [ 18 O]- and[ 16 O]N 5 -formyltetrahydrofolate in in the presence of [ 16 O]H 2 O and using[...
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