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Although there are some commercial vaccines available against infectious pancreatic necrosis virus (IPNV), the disease still continues to be a major problem for aquaculture development worldwide. In the current work, we constructed a DNA vaccine against IPNV (pIPNV-PP) by cloning the long open reading frame of the polyprotein encoded by the viral RNA segment A. In vitro, the vaccine is properly translated...
Coinfection by infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV), which results in the inhibition of IHNV, has been previously characterised in salmonid fish. In the current work, we have studied whether this coinfection has repercussions in the early immune response by studying the expression of two pro-inflammatory cytokines, interleukin 1β (IL-1β) and...
In the present study, six diagnostic methods for the detection of infectious pancreatic necrosis virus (IPNV) (indirect immunofluorescence, flow cytometry, immunoperoxidase, immunodot blot, immunostaphylococcus-protein A, and RT-PCR) have been comparatively evaluated using the seroneutralization as the reference assay, and 83 Spanish isolates and 3 reference strains. The most reliable methods were...
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