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We constructed and tested a series of cloning vectors designed to facilitate protein production and purification indictyostelium discoideum (Dd). These vectors carry the origin of replication of the Dd high-copy-number plasmid Ddp2, expression cassettes consisting of the strong, constitutive actin (act15) or the inducible discoidin (disIγ) promoters, a translational start codon upstream from a multiple...
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