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The α,β2,μ2,σ2 heterotetrameric AP2 complex is recruited exclusively to the phosphatidylinositol-4,5-bisphosphate (PtdIns4,5P 2 )-rich plasma membrane where, amongst other roles, it selects motif-containing cargo proteins for incorporation into clathrin-coated vesicles. Unphosphorylated and μ2Thr156-monophosphorylated AP2 mutated in their αPtdIns4,5P 2 , μ2PtdIns4,5P 2 , and...
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