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To compare the fermentation characteristics of d-lactate, l-lactate and dl-lactate mixture by fecal microbiota of pigs, in vitro fermentation test was conducted with d-lactate, l-lactate and dl-lactate mixture as substrates, feces of piglets as inocula. The concentrations of lactate and short chain fatty acid (SCFA) were quantified during the fermentation. The composition of bacterial communities...
16S ribosomal RNA (rRNA) gene based PCR/denaturing gradient gel electrophoresis (DGGE) and real-time PCR were used to monitor the changes in the composition of microbiota in the hindgut of piglets after oral administration of Lactobacillus sobrius S1. Six litters of neonatal piglets were divided randomly into control group and treatment group. At 7, 9, and 11 days of age, piglets in the treatment...
The effect of feeding whole crop rice (WCR) to growing-finishing pigs at three levels 0 (Control), 10% and 20% on bacterial communities in colon content and feces was analyzed using 16S rDNA-based techniques. Amplicons of the V6–V8 variable regions of bacterial 16S rDNA were analyzed by denaturing gradient gel electrophoresis (DGGE), cloning and sequencing. The total number of DGGE bands and Shannon...
In vitro fermentation of sugar beet pulp (SBP) was carried out to determine which bacterial species would be enriched by use of this carbohydrate source. Faeces from four weaning piglets as a source of inoculum was also compared. The microbial diversity of the prominent bacteria before and after this in vitro fermentation was analysed using denaturing gradient gel electrophoresis (DGGE) of PCR amplicons...
The growth characteristics of 17 isolates of anaerobic fungi from various animal hosts were investigated relative to their ability to degrade wheat straw. The isolates consisted of 11 Neocallimastix isolates from Malaysia, twoPiromyces isolates from Australia and Neocallimastix hurleyensis, two polycentric isolates and aCaecomyces isolate, all from the U.K. These fungi were grown on wheat straw in...
The gut anaerobic fungi,Neocallimastix hurleyensisand aOrpinomyces sp., were grown in 100 mL batch and continuous-flow cultures on wheat straw at a concentration of 80 g dry matter/L of culture liquid. In batch cultures,N. hurleyensisandOrpinomyces sp. degraded only ca. 9% and 5% of the wheat straw, respectively. In continuous-flow cultures, however, the two fungi degraded 52–56% of the apparent dry...
The anaerobic fungus,Neocallimastix hurleyensis, was grown in 100mL batch and continuous-flow cultures on wheat straw concentrations ranging from 5-80g dry matter/L of culture fluid. In batch culture,N. hurleyensiscould degrade about 45% of the wheat straw, but only if the substrate was provided at 5g dry matter/L. In cultures containing 10-80g dry matter/L, progressively less of the straw was degraded...
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