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Fluorine NMR paramagnetic relaxation enhancement was evaluated as a versatile approach for extracting distance information in selectively F‐labeled proteins. Proof of concept and initial applications are presented for the HIV‐inactivating lectin cyanovirin‐N. Single F atoms were introduced at the 4‐, 5‐, 6‐ or 7 positions of Trp49 and the 4‐position of Phe4, Phe54, and Phe80. The paramagnetic nitroxide...
NMR‐Spektroskopie. In der Zuschrift auf S. 158 ff. berichten A. M. Gronenborn und E. Matei über paramagnetische 19F‐Relaxationsverstärkungen, die sie auswerten, um Abstandsinformationen in einem fluorierten Protein zu gewinnen.
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