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Minicircle (MC) DNA vectors have shown prolonged expression in gene transfection studies. Here we have developed a facile approach based on enzyme-catalyzed reactions to produce the MC DNA in vitro. eGFP plasmid was inserted by two mirror-symmetry pairs of EcoRV and HindIII restriction enzyme sites at both sides of the expression cassette. The highly purified eGFP MC DNA vector was obtained through...
Multiple sclerosis (MS) is characterized by the presence of inflammatory demyelinating foci throughout the brain and spinal cord, accompanied by axonal and neuronal damage. Although inflammatory processes are thought to underlie the pathological changes, the individual mediators of this damage are unclear. In order to study the role of pro-inflammatory cytokines in demyelination in the central nervous...
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