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A procedure was proposed for quantification of Fe, Cu and Zn in protein bands using synchrotron radiation X-ray fluorescence (SRXRF) after isoelectric focusing (IEF). Calibration standards were prepared by adding certain amounts of metal ions and free-metal proteins to electrophoresis gel. The calibration curves were obtained in the range of 0–8 μmg/g metals, and linear relationship was observed between...
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