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Three novel reagents have been prepared to introduce photoactivatable o-nitrobenzyl phosphate esters. The use of fluorenylmethyl and t-butyl protecting groups allowed for a wide range of chemical transformations after phosphorylation. In addition, the use of S-acetylthioethyl and acyloxymethyl groups resulted in photo- and bioactivatable phosphate triesters of phosphatidic acid.
The total synthesis of an enantiomerically pure Ins(1,3,4,5)P 4 derivative equipped with a photosensitive nitroveratryl group at the 3-O-phosphate is reported.
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