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L-edge X-ray absorption spectroscopy has been used to study the electronic structure of Ni in the Ni–Fe hydrogenases and CO-dehydrogenases under a variety of conditions. The L-edge spectra are interpreted by comparison with the spectra of Ni model complexes and by ligand field multiplet simulations to examine the Ni oxidation and electronic spin states. The spectra for Ni in oxidized Desulfovibrio...
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