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Current research has shown that different sites of the human body house different bacterial communities. There is a strong correlation between an individual's microbial community profile at a given site and the onset of disease. Chronic Obstructive Pulmonary Disease (COPD) is a progressive lung disease resulting in narrowing of the airways and restricted airflow. Despite being the third leading cause...
Current methods of understanding microbiome composition and structure rely on accurately estimating the number species and their relative abundance. Most of these methods require an efficient PCR whose forward and reverse primers bind well to the same, large number of identifiable species, and produce amplicons that are unique. It is therefore not surprising that currently used universal primers designed...
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