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Acinetobacter baumannii is a species of non fermentative Gram-negative bacteria commonly found in water and soil. This organism was susceptible to most antibiotics in the 1970s. It has now become a major cause of hospital-acquired infections worldwide due to its remarkable propensity to rapidly acquire resistance determinants to a wide range of antibacterial agents. Herein we have determined the...
TheAspergillus ficuum phytase genephyA was overexpressed inPichia pastoris X33 after replacing Buffered glycerol-complex medium (BMGY medium) using 1% (v/v) glycerol with fresh Buffered methanol-complex medium (BMMY medium) using 1% (v/v) methanol (on daily basis) as carbon sources. The phytase activity increased evidently with the induction time, and reached 200 U mL−1 after 9 days of induction....
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