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An electrochemiluminescence (ECL) based biosensor is described for determination of microRNAs in the A549 cell line. Firstly, graphene oxide (GO) is dripped onto a glassy carbon electrode surface to form an interface to which one end of the capture probe (with a stem-loop structure) can be anchored through π-interaction via dangling unpaired bases. The other end of the capture probe is directed away...
Here, we present a dual amplification strategy based on a duplex-specific nuclease and a hybridization chain reaction, which has been designed for the highly sensitive detection of miRNAs. In the first step, a loop amplification strategy based on a duplex-specific nuclease was employed to obtain a large number of ssDNA reporters that then act as initiators to trigger the next amplification step. The...
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