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Abstract: Escherichia coli O157:H7 contamination of leafy green vegetables is an ongoing concern for consumers. Biofilm‐associated pathogens are relatively resistant to chemical treatments, but little is known about their response to irradiation. Leaves of Romaine lettuce and baby spinach were dip inoculated with E. coli O157:H7 and stored at 4 °C for various times (0, 24, 48, 72 h) to allow biofilms...
In nature, bacteria are often organized in aggregates or biofilms rather than as solitary cells. Biofilms on inert surfaces have been studied in depth using confocal scanning laser microscopy (CSLM) with a variety of fluorescent probes. In contrast, there have been few studies of native microbial aggregates or biofilms on living surfaces, including plants. In this study we used CSLM in combination...
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