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The PNA–DNA interaction was probed with single-molecule AFM force measurements. The PNA sequence with only six thymine bases, p(T)6, could form stable hybrids with the complementary DNA sequence of d(A)6. Rupture of the p(T)6–d(A)6 hybrids required forces of around 148pN, which was larger than the forces to unbind short DNA duplex that were usually below 100pN. Fitting into the rupture force–loading...
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