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Positive transcription elongation factor b (P‐TEFb), the complex of Cyclin T1 and CDK9, activates the transcription of many viral and eukaryotic genes at the point of mRNA elongation. The activity of P‐TEFb has been implicated in the differentiation of a number of cell types, including skeletal muscle. In order to promote transcription, P‐TEFb hyperphosphorylates RNA Pol II, thereby increasing its...
The wild‐type Escherichia coli RecA protein is a recombinase platform with unrealized recombination potential. We have explored the factors affecting recombination during conjugation with a quantitative assay. Regulatory proteins that affect RecA function have the capacity to increase or decrease recombination frequencies by factors up to sixfold. Autoinhibition by the RecA C‐terminus can affect recombination...
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