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Tyrosine-83 in spinach plastocyanin (Pc) has been modified by site-directed mutagenesis to a histidine. An NMR titration yields a pK value of 8.44 for this residue. The high value is probably due to the acidic residues close to this site. The reduction potential is increased by 35 mV at pH 7.5, but only slightly, if at all, at pH 8.9. EPR and optical absorption bands associated with the copper...
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