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An M intermediate of wild-type bacteriorhodopsin and an N intermediate of the V49A mutant were accumulated in photostationary states at pH 5.6 and 295 K, and their crystal structures determined to 1.52A and 1.62A resolution, respectively. They appear to be M 1 and N' in the sequence, M 1 M 2 M' 2 N N'->O->BR, where M 1 , M 2 , and M' 2 ...
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