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Tropomyosin is present in virtually all eucaryotic cells, where it functions to modulate actin-myosin interaction and to stabilize actin filament structure. In striated muscle, tropomyosin regulates contractility by sterically blocking myosin-binding sites on actin in the relaxed state. On activation, tropomyosin moves away from these sites in two steps, one induced by Ca 2+ binding to...
Calcium-dependent regulation of tension and ATPase activity in permeabilized porcine ventricular muscle was lost after incubation with 10 mM vanadate. After transfer from vanadate to a vanadate-free, low-Ca 2+ solution (pCa > 8), the permeabilized muscle produced 84.8% +/- 20.1% (+/- S.D., n=98) of the isometric force elicited by high Ca2 2+ (pCa ~4.5 prior to incubation...
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