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Weinvestigated the folding, stability, and specificity of dimerization of the neck-hinge region (residues 356–416) of the kinesin-like protein Kif3A. We showed that the predicted coiled-coil on its own (residues 356–377) will fold autonomously in solution. We then explored the ability of oppositely charged regions to specify heterodimer formation in coiled-coils by synthesizing analogs of the neck...
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