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Binding of small RNAs by the RNA-dependent RNA polymerase of coliphage Qβ was studied utilizing a fluorometric assay. A DNA oligonucleotide probe of sequence 5′-d(TTTTTCC) was 5′-end-labeled with pyrene. In this construct, the proximal thymine residues efficiently quench the fluorophore emission in solution. Upon stoichiometric binding of one probe per polymerase molecule, the pyrene steady-state...
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