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In nucleic acid polymerization reaction, pyrophosphorolysis is the reversal of nucleotide addition, in which the terminal nucleotide is excised in the presence of inorganic pyrophosphate (PPi). The CCA enzymes are unusual RNA polymerases, which catalyze CCA addition to positions 74–76 at the tRNA 3′ end without using a nucleic acid template. To better understand the reaction mechanism of CCA addition,...
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