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Enterolactone (ENL) is produced by the gut microflora from lignans found in edible plants. ENL is estrogenic with no effect on the E-screen test and is a natural Selected Estrogen Receptor Modulator (SERM) with health interests that have to be checked in clinical studies with bioavailability assessment. Two haptens of ENL were synthesized, with a spacer arm at the C5 position having either 2 or 4...
Carboxylic acid haptens were synthesised, for Hesperetin (Hesp) and Naringenin (Nar). They had a spacer arm (4 or 6 carbons long) on the C7 position. Haptens were coupled to bovine serum albumin. Polyclonal antibodies were generated. Enzyme-linked immunosorbent assays (ELISAs) were developed. Owing to sensitivity, one antibody for each flavanone was retained, namely anti-h4-Hesp and anti-h4-Nar. For...
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