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The temperature and pH dependence as well as the selectivity of the peroxidase activity of a complex associating a monoclonal antibody 13G10 with its iron(III)-α,α,α,β-meso-tetrakis(ortho-carboxyphenyl) porphyrin (Fe(ToCPP)) hapten have been studied and compared to those of Fe(ToCPP) alone. It first appears that the peroxidase activity of the 13G10-Fe(ToCPP) complex is remarkably thermostable and...
In order to get catalytic antibodies modelling peroxidases BALB/c mice have been immunized with iron(III)-α,α,α,β-mesotetrakis-orthocarboxyphenyl-porphyrin (Fe-(ToCPP))-KLH conjugates. Monoclonal antibodies have been produced by the hybridoma technology. Three antibodies, 2 IgG 1 and 1 IgG 2a , were found to bind both Fe(ToCPP) and the free base ToCPPH 2 with similar...
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