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The nucleic acid analog 2-aminopurine (2AP) can be site-specifically incorporated into oligonucleotides without significantly affecting the thermal stabilities of DNA DNA duplexes. Because the absorption band of 2AP extends beyond 300 nm, it can be selectively ionized with intense 308-nm excimer laser pulses by a tandem two-photon absorption mechanism yielding site-specifically positioned 2AP radical...
The nucleic acid analog 2-aminopurine (2AP) can be site-specifically incorporated into oligonucleotides without significantly affecting the thermal stabilities of DNADNA duplexes. Because the absorption band of 2AP extends beyond 300 nm, it can be selectively ionized with intense 308-nm excimer laser pulses by a tandem two-photon absorption mechanism yielding site-specifically positioned 2AP radical...
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