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Adenosine deaminase (ADA, EC 3.5.4.4) is a ubiquitous (β/α) 8 -barrel enzyme crucial for purine metabolism and normal immune competence. In this study, it was observed that loss of enzyme activity of murine ADA (mADA) precedes the global secondary and tertiary structure transition when the protein is exposed to denaturant. The structural mechanism for this phenomenon was probed using site-specific...
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