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Toward a complete LC-MS mapping system for peptides and proteins, we have coupled a precision-flow microbore HPLC system to electrospray single quadrupole mass spectrometer. The HLPC system allows fast separation of protein digests with UV detection at the low pmol level. A μl/min portion (1:25) of the effluent is passed into a high-sensitivity electrospray MS system. The electrospray source allows...
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